Effect of Gubao Capsule on Bone Mineral Density and Bone Microstructure in Rats with Glucocorticoid-Induced Osteoporosis
Objective:To observe the effect of Gubao Capsule on bone mineral density and bone microstructure in rats with glucocorticoid-induced osteoporosis.Methods:Forty male Wistar rats were randomly divided into blank group,model group,drug administration group(Gubao Capsule)and control group(Caltrate D),with 10 rats in each group.The model group,drug administration group and control group were injected with dexamethasone sodium phosphate(2.5 mg/kg),and the blank group was injected with 0.9%sodium chloride(2.5 mL/kg)twice a week(Monday and Thursday)for 8 weeks.After successful modeling,the drug administration group was gavaged Gubao Capsules[0.4 g/(kg·d)],the control group was gavaged Caltrate D 60 mg/(kg·d),and the blank group and model group were gavaged the same volume of normal saline,once a day.Intraperitoneal injection of 2%pentobarbital sodium was administered after 12 weeks of gastric lavage and the rats were euthanized.Centrifuge serum was obtained and serum calcium,serum phosphorus and alkaline phosphatase levels were determined.Computed tomography(CT)was done for the left femur of rats by Micro-CT and the image of the region of interest was reconstructed in 3D to observe and analyze the skeletal conditions of each group.Hematoxylin-eosin staining was used to observe the histopathological changes of right femur specimens.Results:The serum biochemical indexes showed that the serum calcium level in the administration group and the control group was significantly higher than that in the model group(P＜0.05).Micro-CT images and 3D reconstruction graphics showed that the bone trabecular structure was sparse in the model group.After the treatment with Gubao Capsule and Caltrate D,BMD,BV/TV,Tb.N increased significantly,and Tb.Sp decreased significantly(P＜0.05).CT and 3D images showed that the improvement of bone mineral density and bone microstructure in the administration group was slightly superior to that in the control group.The results of hematoxylin-eosin staining showed that the bone trabeculae were sparse,fractured and separated in the model group,while the number of bone trabeculae increased and the structure was dense in the drug administration group and the control group.Conclusion:Gubao Capsule can improve osteoporosis by affecting bone mineral density,bone microstructure and bone metabolism in glucocorticoid rats.
OsteoporosisGlucocorticoidsGubao CapsuleBone mineral densityBone metabolism
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NSTL
万方数据
维普
