Antidepressant Activity of Sharpleaf Glangal Fruit Alcohol Extract
Objective:To study the antidepressive activity of sharpleaf glangal fruit,Alpiniae Oxyphyllae Fructus alcohol extract(AOY),and preliminary clarify its mechanism of action.Methods:Mouse ear swelling test and mouse cotton ball granuloma test were used to investigate the anti-inflammatory ability of AOY;the antioxidant capacity of AOY was investigated by using the antioxidant model of DPPH free radical,ABTS free radical,hydroxyl free radical and superoxide anion free radical scavenging power in vitro;a mouse model of chronic unpredictable mild stress(CUMS)was used to investigate its antidepressant activity by forced swimming test(FST),sugar preference test(SPT)and tail suspension test(TST).The action mechanism of AOY was explored by measuring the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),nuclear transcription factor(NF-κB),inflammatory body(NLRP3)and interleukin-1β(IL-1β)in the hippocampus of mice.Results:Alcohol extract of AOY inhibits acute and chronic inflammation in mice.AOY was comparable to the antioxidant level of vitamin C(VC)at a certain concentration,indicating that it has good antioxidant activity.AOY could significantly enhance the sugar preference degree of mice in the sugar preference experiment,and significantly reduce the immobile time of mice in the tail suspension experiment and the forced swimming experiment.After AOY administration,SOD and GSH-Px activities increased in the hippocampus of mice,while iNOS,COX-2,NF-κB,NLRP3 and IL-1β contents decreased.Conclusion:Sharpleaf glangal fruit has good anti-inflammatory and antioxidant activity,and AOY can act on both inflammatory response and oxidative stress pathways to play an antidepressant role.
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