Effect of Xuanbai Chengqi Decoction on Pathological Damage and Th17/Treg Imbalance of Lung Tissue of Asthmatic Rats with Phlegm-Heat and Fu-Organ Excess
Objective:To observe the effect of Xuanbai Chengqi Decoction(XCD)on pathological damage of lung tissue and imbalance of Th17/Treg in broncho-alveolar lavage fluid(BALF)in asthmatic rats with phlegm-heat and fu-organs excess.Methods:Fifty male Wistar rats were randomly divided into Group A(blank control group),Group B(model group),Group C(Dingchuan decoction(DCD)Group),Group D(XCD Group)and Group E(dexamethasone Group),with 10 rats in each group.Asthma model was induced by OVA and the factors of phlegm-heat were applied to establish the model.After the model was established successfully,Groups C,D,and E were given DCD(0.067 g/mL),SCD(0.036 g/mL),and dexamethasone solution(0.05 mg/100 g/d)at a dose of 1.5 mL/100 g,respectively;while groups A and B were given physiological saline in equal proportion once a day.After 14 days of continuous medication,the general conditions of rats in each group were observed.The pathological changes of the lung tissue were observed by HE staining,and the contents of IL-6,transforming growth factor-β(TGF-β),IL-17 and IL-10 in broncho-alveolar lavage fluid were compared by enzyme-linked immunosorbent assay(ELISA).Results:Pathological section results showed that no inflammatory cell infiltration was observed in group A,alveolar dilation was good,and bronchial mucosa was normal.A large number of eosinophilic granulocyte and lymphocyte infiltration were found in group B.The inflammatory infiltration of cells in groups C,D and E were reduced,and the pathological conditions were improved.ELISA results showed that compared with group A,the levels of IL-6 and IL-17 in alveolar lavage fluid in group B significantly increased(all P<0.01).Compared with group B,IL-6 and IL-17 levels in groups C,D and E decreased(all P<0.01).Compared with group A,the levels of TGF-β and IL-10 in alveolar lavage fluid in group B decreased(all P<0.01).Compared with group B,the levels of two cytokines in group D significantly increased(all P<0.01).The level of TGF-β in group C increased(P<0.05),and the level of IL-10 significantly increased(P<0.01).The level of IL-10 in group E significantly increased(P<0.01),but the level of TGF-β did not significantly increase(P>0.05).Conclusion:Xuanbai Chengqi Decoction can treat asthma of phlegm heat and fu-organ excess type through the method of discharge heat and unblocking fu-organs,improving asthma symptoms,alleviating pathological damage to rat lung tissue,and changing the contents of IL-6,TGF-β,IL-17,and IL-10 in alveolar lavage fluid,thus,regulating the imbalance of Th17/Treg.
Xuanbai Chengqi DecoctionPhlegm-heat and fu-organ excessAsthmaPathological changesTh17/Treg
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