Objective:To explore the best stir-baking with salt of Achyranthis Bidentatae Radix.Methods:In order to optimize stir-baking with salt of Achyranthis Bidentatae Radix,the technique of ultra high performance liquid chromatography tandem mass spectrometry was adopted;five effective ingredients for tonifying kidney and strengthening bone of Achyranthis Bidentatae Radix,β-Ecdysterone,25R Achyranthes,25S Achyranthes,Ginsenoside R0,and Bamboo Joint Ginsenoside Iva,were taken as evaluation indicators;single factor and Box Behnken design methods was employed;and the amount of salt added,frying temperature,and frying time were the factors to be investigated.Results:The best technique of stir-baking with salt of Achyranthis Bidentatae Radix was that the amount of salt is 2%,stirring temperature 100℃,stirring time 20 min.Conclusion:The ingredients of stir-baking with salt of Achyranthis Bidentatae Radix are stable and reliable,which is beneficial to the quality uniformity of Achyranthis Bidentatae Radix processed with salt.
关键词
牛膝/Box-Behnken响应面法/盐炙/有效成分/超高效液相色谱串联质谱
Key words
Achyranthis Bidentatae Radix/Box-Behnken design-response surface methodology/Stir-baking with salt/Effective components/Ultra high performance liquid chromatography tandem mass spectrometry
维普
万方数据