Exploring the Mechanism of Liyan Formula in the Treatment of Chronic Pharyngitis Based on Network Pharmacology and Animal Experiments
Objective:To explore the mechanism of Liyan Formula in the treatment of chronic pharyngitis based on network pharmacology and animal experiments.Methods:The active components and targets of Liyan Formula were collected from TCMSP database and related targets of chronic pharyngitis were collected from the GeneCards database.Intersection targets were obtained through Venny and String websites,and KEGG pathway analysis and Gene Ontology enrichment analysis were performed on the intersection targets.Tongg ammonia spray was used to establish a rat model of chronic pharyngitis,and the results of network pharmacology were verified.Results:141 active compounds,205 action targets and 1 950 disease targets were collected on Liyan Formula,and 112 targets were obtained by intersection analysis.Important targets such as IL-6,TNF and IL-β were obtained by PPI network analysis of intersection targets.KEGG pathway showed that the pathway with high correlation with Liyan Formula in treating chronic pharyngitis was cancer-related pathway.The results of GO enrichment analysis showed that the biological process of the therapeutic effect of Liyan Formula mainly involved the reaction of inorganic substances.The cell components mainly involved positions such as the membrane raft,the outer membrane of the organelle,and the cyst cavity.The molecular functions were mainly related to cytokine receptor binding and DNA transcription factor binding.The results of animal experiments showed that Liyan Formula could effectively reduce the levels of TNF-α,IL-1β and IL-6 in serum,and reduce the levels of TLR-4,NF-κB P65 and phosphorylated I-κBα protein in rat throat mucosa.Conclusion:The active components of Liyan Formula and its potential targets for the treatment of chronic pharyngitis are predicted through network pharmacology.Meanwhile,the animal experiments showed that Liyan Formula can treat ammonia-induced chronic pharyngitis in rats by regulating TLR-4/NF-κB signaling pathway.
万方数据
维普
