Effects of Taurine on Cardiac Tissue Distribution of Toadstene
Objective:To investigate the effect of taurine(Tau)on the cardiac tissue distribution of toadstene.Methods:Sixteen male guinea pigs were randomly divided into blank group,model group(8 mg/kg toad venom),low dose taurine group(8 mg/kg toad venom + 150 mg/kg Tau)and high dose taurine group(8 mg/kg toad venom + 300 mg/kg Tau).After corresponding treatment,HPLC-ESI-TOF-MS was used to analyze the uptake of toadstene in the hearts of guinea pigs in different administration groups,and molecular docking technology was further used to simulate the binding site of taurine and SCN5A,a known target protein related to heart disease.Results:Nine types of toadstenes,Gtl,Arg,Hel,Tel,Btl,Bul,Rbg,Cbg and Cbt,were detected in the heart of the model group.Some metabolites that might have been undergone hydroxylation were detected in the liver and kidney.The prototype compounds related to toadstene were also detected in the hearts of the taurine groups,but the intake of toadstene in the heart was significantly reduced compared with the model group.The binding energy of molecular docking simulated taurine and SCN5A was-3.7 kcal/mol,and taurine could be hydrogen bonded to the receptor amino acids Glu1225,Arg1306,Arg1309.Conclusion:Taurine can delay the uptake of toadstene components in the heart of the target organ,thereby reducing the cardiotoxicity of toad venom.It is speculated that the binding check point of taurine and SCN5A may be Glu1225,Arg1306,and Arg1309.This finding provides a practical experimental basis for the application of traditional Chinese medicine compatibility in the treatment of heart diseases.
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