Effect of Danlong Xingnao Formula on Cognitive and Hippocampal Pathology of Vascular Dementia Rats with Kidney Deficiency and Blood Stasis Syndrome
Objective:To establish the animal model of vascular dementia(VD)disease with kidney deficiency and blood stasis syndrome,and observe the effects of Danlong Xingnao Formula(DXF)on cognitive function and hippocampal pathology of the animal model.Methods:Seventy-two SD rats were randomly divided into sham operation group(n=12)and model group(n=60).In the model group,60 rats were fed with high-fat diet combined with modified 2-VO method and sexual organ removal method to establish the model of VD with kidney deficiency and blood stasis syndrome.After successful modeling,the rats were randomly divided into model group,nimodipine group and DXF high-dose,medium-dose and low-dose groups,and the animals were administered for 4 weeks.The changes of estradiol(E2),testosterone(T),osteocalcin(OCN),calcium(Ca),hemorheology,endothelin-1(ET-1),nitric oxide(NO)and other indexes were observed in the sham operation group and model group.Behavioral testing was conducted using the Morris water maze experiment.HE staining was conducted for the hippocampus of the brain.Results:Characteristics of kidney deficiency and blood stasis syndrome model with typical VD were visible in the successful modeling rats.In the model group,the levels of E2,T,OCN,Ca and NO were significantly lower than those in the sham group,while the whole blood viscosity(low,medium and high shear)and ET-1 level were significantly higher than those in the sham group,the escape latency was significantly longer than that of the sham group,and the number of crossing platforms and the target quadrant residence time were significantly shorter than those of the sham group(P<0.01).Compared with the model group,the DXF groups and nimodipine group had significantly shorter escape latency and longer crossing platforms and target quadrant residence time(P<0.05,P<0.01).The pathological status of the hippocampal CA1 area in rats treated with various doses of DXF and nimodipine improved.Conclusion:The model rats constructed by combining high-fat diet feeding with improved 2-VO and genital organ removal method met the characteristics of VD with kidney deficiency and blood stasis syndrome.Danlong Xingnao Formula can improve the cognitive ability of rats with VD and kidney deficiency and blood stasis syndrome,improve the pathological morphology of the hippocampus,and play a role in improving VD with kidney deficiency and blood stasis syndrome.
Danlong Xingnao FormulaVascular dementiaCognitive functionHippocampusKidney deficiency and blood stasis syndrome
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