Effect of Scutellariae Radix on Zebrafish Anxiety and Molecular Docking Analysis Targeting GABA Receptor
Objective:To investigate the therapeutic effects and potential mechanisms of the 50%ethanol extract of Scutellariae Radix on zebrafish anxiety induced by 1-(3-chlorophenyl)piperazine hydrochloride(mCPP).Methods:In the anxiolytic study,zebrafish at 10 and 30 days post-fertilization were divided into control group,model group(0.1 nmol/L mCPP),and treatment groups(different concentrations of Scutellariae Radix extract+0.1 nmol/L mCPP).Zebralab software was used to analyze the average movement distance of the zebrafish.Molecular docking software Autodock Vina was employed to analyze the binding energy between 12 main components of Scutellariae Radix and the gamma-aminobutyric acid A receptor(GABAAR),identifying potential active components.Results:The 50%ethanol extract of Scutellariae Radix inhibited mCPP-induced anxiety in zebrafish at both 10 and 30 days post-fertilization.For zebrafish at 30 days post-fertilization,the average movement distances for the control group,model group(0.1 nmol/L mCPP),and treatment group(200 μg/mL+0.1 nmol/L mCPP)were 3,633 mm,4,280 mm,and 3,894 mm,respectively.This indicates that 200 μg/mL of Scutellariae Radix extract can alleviate the anxiety behavior induced by 0.1 nmol/L mCPP in 30-day-old zebrafish.Additionally,the anxiety behavior of the zebrafish conformed to thigmotaxis.Eleven active components of Scutellariae Radix had stronger binding energies with GABAAR compared to the original ligand diazepam(-7.2 kcal/mol),with the strongest being oroxylin A(-8.8 kcal/mol).The main components baicalein,baicalin,wogonin,and wogonoside also showed strong binding energies of-8.3,-7.7,-8.3,and-7.3 kcal/mol,respectively.It is predicted that flavonoids such as baicalin are the primary substances for Scutellariae Radix's anxiolytic effects.Conclusion:Scutellariae Radix exhibits a definitive anxiolytic effect on zebrafish,and its active mechanism may be related to the activation of GABAAR by flavonoid components of Scutellariae Radix.
Scutellariae RadixZebrafishAnxietyMolecular dockingGamma-aminobutyric acid a receptor
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