Effect of Yifei Tongluo Formula on Epithelial-Mesenchymal Transition in Pulmonary Fibrosis Rats
Objective:To explore the mechanism of Yifei Tongluo Formula intervention in pulmonary fibrosis(PF)in rats.Methods:Fifty-six male SD rats were randomly divided into 10 rats for the blank group(control group),and the remaining 56 rats were intratracheally instilled with bleomycin sulfate(5 mg/mL)to establish a PF animal model.After successful modeling,the surviving 40 rats were randomly divided into the model group,prednisone acetate group(PRED group),low-dose Yifei Tongluo Formula group(YFTL-L group),and high-dose Yifei Tongluo Formula group(YFTL-H group).The PRED group was gavaged with 1.17 mg/kg prednisone acetate tablets,and the YFTL-L group and YFTL-H group were gavaged with 1.027 and 2.054 g/mL Yifei Tongluo Formula decoction,respectively.The control group and model group were gavaged with an equal volume of physiological saline.The drugs were administered continuously for 21 days.After treatment,the lung coefficient was calculated;HE and Masson staining were used to observe lung tissue damage and collagen deposition;ELISA was used to detect serum hydroxyproline(Hyp)and TNF-α levels;immunofluorescence was used to detect the protein levels of TGF-β1,Col-I,and E-cadherin in lung tissue;immunohistochemistry was used to detect the protein levels of α-SMA and vimentin in lung tissue.Results:Compared with the model group,the lung tissue structure damage in the YFTL-L group and YFTL-H group was improved,collagen deposition was reduced,the lung coefficient and fibrosis score decreased(P＜0.01),serum levels of Hyp and TNF-α decreased(P＜0.01),the protein expression of TGF-β1,Col-I,α-SMA,and vimentin in lung tissue was downregulated(P＜0.01,P＜0.05),and E-cadherin protein levels increased(P＜0.05,P＜0.01).Moreover,there was a certain dose-dependent effect with increasing drug concentration.Conclusion:Yifei Tongluo Formula can delay the progression of PF in rats,and its mechanism of action may be through alleviating pulmonary inflammation and inhibiting epithelial-mesenchymal transition to exert anti-fibrotic effects.
万方数据
维普
