Exploring the Mechanism of Banxia Xiexin Decoction in Repairing Intestinal Mucosal Barrier Based on p38 MAPK/NF-κB/MLCK Signaling Pathway
Objective:To explore the mechanism by which Banxia Xiexin Decoction(BXD)inhibits intestinal inflammatory response and repairs the intestinal mucosal barrier in vivo.Methods:Thirty-six female SPF-grade SD rats were randomly divided into a blank group,a model group,a Western medicine group(mesalazine group),and low,medium,and high-dose Banxia Xiexin Decoction groups(BXD-low,BXD-medium,and BXD-high dose groups).After 7 days of adaptive feeding,all groups except the blank group were freely given 5%DSS for 7 days to induce an ulcerative colitis rat model.Following successful modeling,the blank and model groups were gavaged with physiological saline,the Western medicine group was gavaged with mesalazine(0.054 g/d),and the BXD groups were gavaged with Banxia Xiexin Decoction extract at doses of 2.205 g/kg,4.41 g/kg,and 8.82 g/kg respectively,for 15 days.The Disease Activity Index(DAI)was used to assess general changes after modeling and drug treatment.Hematoxylin-eosin(HE)staining confirmed the extent of colonic tissue damage.ELISA detected changes in the expression of inflammatory factors in serum,and qRT-PCR and Western blot were used to detect the expression of key proteins in the p38 MAPK/NF-κB/MLCK signaling pathway.Results:The 5%DSS-induced ulcerative colitis model rats showed weight loss,diarrhea,bloody stools,and shortened colons,confirming successful modeling.After administration of BXD and mesalazine,DAI scores decreased to varying degrees.HE results indicated that mesalazine and high-dose BXD significantly improved DSS-induced pathological changes in rat colonic tissue.ELISA results suggested that high-dose BXD and mesalazine significantly inhibited the expression levels of pro-inflammatory factors,thereby achieving an anti-inflammatory effect in the intestines.Western blot and qPCR results showed that BXD could repair the intestinal mucosal barrier by regulating the expression of MAPK,MLCK,NF-κB,and Occludin.Conclusion:Banxia Xiexin Decoction can reduce intestinal mucosal permeability and repair damaged intestinal mucosa,potentially through the inhibition of the p38 MAPK/NF-κB/MLCK signaling pathway.
万方数据
维普
