Analysis of the Diuretic Mechanism of Malva Fructus Alcohol Extract Based on the Axis of Gut Microbiota and Renal Tissue Metabolites
Objective:To investigate the diuretic mechanism of the alcohol extract of Malva Fructus through the analysis of renal metabolites and gut microbiota.Methods:Fifty Sprague-Dawley rats were randomly divided into five groups:a normal group(saline),high,medium,and low dose groups of alcohol extract of Malva Fructus(1.6069,0.5356,0.1785 g/kg),and a positive control group(hydrochlorothiazide 0.0214 g/kg).Metabolomics was used to detect endogenous metabolites in the kidneys and characterize metabolic differences,identifying differential metabolites.16S rDNA sequencing was used to assess the diversity and composition of gut microbiota across different dosage groups of the alcohol extract,providing a comprehensive evaluation of its diuretic mechanism.Results:Metabolomics analysis showed that compared to the normal group,the low dose group of Malva Fructus alcohol extract had 13 differential metabolites,the medium dose group had 28,and the high dose group had 14,all showing significant changes in levels.The Venn diagram based on OTU data revealed that compared to the normal group,the Malva Fructus alcohol extract dosage groups shared 2,146 OTUs,with the low dose group having 416 unique OTUs,the medium dose group 381 unique OTUs,and the high dose group 330 unique OTUs.Alpha diversity indicated that the gut microbiota abundance in the low and medium dose groups was higher than in the normal group,while the high dose group had lower abundance,suggesting a regulatory effect of Malva Fructus on gut microbiota in normal rats.At the phylum,genus,and family levels,there were significant differences in relative abundance.Compared to the normal group,the Firmicutes increased and Bacteroidetes decreased across the Malva Fructus alcohol extract dosage groups.At the genus and family levels,Lactobacillus decreased in the low and high dose groups,while Ruminococcaceae increased across all dosage groups.Conclusion:The diuretic mechanism of Malva Fructus may be related to the pathways involving Bacteroidetes-glucose/gluconic acid and Ruminococcaceae-L-tyrosine,among other endogenous metabolites and gut microbiota pathways.
Gut microbiotaRenal tissueMetabolitesAlcohol extract of Malva FructusMechanism analysis
万方数据
维普
