Effects of Catalpol of Rehmannia Glutinosa on the PI3K/Akt/mTOR Signaling Pathway Following Ischemia-Reperfusion Injury in Rats
Objective:To explore the neuroprotective effects of Catalpol of Rehmannia Glutinosa based on the PI3K/Akt/mTOR signaling pathway in rats with brain ischemia-reperfusion injury.Methods:Thirty-six male SPF-grade SD rats were randomly divided into three groups:Sham surgery group,Model group,and Catalpol of Rehmannia Glutinosa group(5 mg/kg),with 12 rats in each group.Except for the Sham surgery group,the Model group and the Catalpol of Rehmannia Glutinosa group underwent a middle cerebral artery occlusion(MCAO)procedure to create a brain ischemia-reperfusion injury model.After the procedure,the Catalpol of Rehmannia Glutinosa group received intraperitoneal injections of Catalpol of Rehmannia Glutinosa in saline solution,while the Sham surgery and Model groups received intraperitoneal injections of saline.Treatments were administered daily for 7 days.Neurological function recovery was assessed using the Longa scoring system.Brain water content was measured using the dry/wet weight method.ELISA was used to measure malondialdehyde(MDA)levels and superoxide dismutase(SOD)activity in the ischemic brain tissue.Western blot analysis was employed to assess the expression of proteins related to the PI3K/Akt/mTOR pathway in the ischemic brain cortex.Results:Compared to the Sham surgery group,the Model group exhibited significant neurological deficits with increased neurological impairment scores(P<0.01).The Catalpol of Rehmannia Glutinosa group showed significantly reduced neurological impairment scores compared to the Model group(P<0.05).Brain water content was significantly higher in the Model group compared to the Sham surgery group,indicating edema(P<0.01).The Catalpol of Rehmannia Glutinosa group had significantly reduced brain water content compared to the Model group(P<0.01).The Model group had decreased SOD levels and increased MDA levels in the ischemic brain tissue compared to the Sham surgery group(P<0.01).The Catalpol of Rehmannia Glutinosa group had significantly increased SOD levels and decreased MDA levels compared to the Model group(P<0.01).Compared to the Sham surgery group,the Model group showed increased levels of p-PI3K,p-Akt,and p-mTOR proteins(P<0.05,P<0.01).The Catalpol of Rehmannia Glutinosa group showed increased levels of p-PI3K/PI3K,p-Akt/Akt,and p-mTOR/mTOR proteins compared to the Model group(P<0.05).Conclusion:Catalpol of Rehmannia Glutinosa significantly improves neurological function,reduces brain edema,and upregulates SOD,p-PI3K/PI3K,p-Akt/Akt,and p-mTOR/mTOR levels while downregulating MDA levels in brain tissue.The neuroprotective effects of Catalpol of Rehmannia Glutinosa are likely related to its ability to activate the PI3K/Akt/mTOR signaling pathway.
Catalpol of rehmannia glutinosaBrain ischemia-reperfusion injuryNeurological functionOxidative stressPI3K/Akt/mTOR signaling pathway
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