Effect of Jianwei Zhixie Mixture on the Expression of Brain Gut Peptides VIP and CCK in Rats with Functional Diarrhea of Spleen Deficiency Type
Objective:To observe the effects of different doses of Jianwei Zhixie Mixture on the expression of cerebral intestinal peptide VIP and CCK in rats with functional diarrhea with spleen deficiency.Methods:50 SPF rats were randomly divided into blank control group,model group,Mommy love group,Jianwei Zhixie Mixture low-dose group and Jianwei Zhixie Mixture high-dose group with 10 rats each.The Mamai group was given 0.2g/(kg·d)intragastric administration,and the low and high dose Jianwei Zhixie Mixture groups were given 4.5 and 18g/(kg·d)Jianwei Zhixie Mixture intragastric administration,respectively,for a total of 14 days.The rats in the model group and the blank control group were given 0.9%NaCl solution freely every day for 14 days.After administration,the rats were killed by neck removal,and the expressions of VIP and CCK in serum were determined by immunohistochemistry.Results:Compared with blank control group,the expressions of VIP and CCK were significantly increased in model group(P<0.05).Compared with the model group,the expression of cerebral intestinal peptide VIP and CCK decreased in the mommy love group.Compared with model group,VIP expression of brain-gut peptide was significantly decreased in low-dose and high-dose Jianwei Zhixie Mixture groups(P<0.05),CCK expression of brain-gut peptide was significantly decreased in high-dose Jianwei Zhixie Mixture group(P<0.05).Conclusion:Jianwei Zhixie Mixture can improve functional diarrhea by reducing the expression of VIP and CCK,regulating intestinal movement and water metabolism in rats with functional diarrhea of spleen deficiency.
Jianwei Zhixie MixtureFunctional diarrhea with spleen deficiencyVIPCCK
万方数据
维普
