中医药信息2024，Vol.41Issue(12) ：23-26,31.DOI:10.19656/j.cnki.1002-2406.20241205

健胃止泻合剂对脾虚型功能性腹泻大鼠脑肠肽VIP、CCK表达的影响

Effect of Jianwei Zhixie Mixture on the Expression of Brain Gut Peptides VIP and CCK in Rats with Functional Diarrhea of Spleen Deficiency Type

武正权 杨红娟 姚柳伊 郭军
中医药信息2024，Vol.41Issue(12) ：23-26,31.DOI:10.19656/j.cnki.1002-2406.20241205
✕
  • 维普
  • 万方数据

健胃止泻合剂对脾虚型功能性腹泻大鼠脑肠肽VIP、CCK表达的影响

Effect of Jianwei Zhixie Mixture on the Expression of Brain Gut Peptides VIP and CCK in Rats with Functional Diarrhea of Spleen Deficiency Type

武正权 1杨红娟 2姚柳伊 1郭军1
扫码查看

作者信息

  • 1. 甘肃省中医院,甘肃 兰州 730050
  • 2. 甘肃中医药大学,甘肃 兰州 730000
  • 折叠

摘要

目的:观察不同剂量健胃止泻合剂对脾虚型功能性腹泻大鼠脑肠肽VIP、CCK表达的影响.方法:50只SPF级Wistar大鼠随机分为空白对照组、模型组、妈咪爱组、健胃止泻合剂低剂量组、健胃止泻合剂高剂量组各10只.除空白对照组外,其余各组大鼠采用灌胃大黄水煎液7.5 g/(kg·d),并每日负重游泳,以力竭为度的方法建立脾虚型功能性腹泻模型.在造模的同时,模型组和空白对照组每天给予大鼠自由饮用0.9%NaCl溶液,健胃止泻合剂高、低剂量组分别灌胃健胃止泻合剂18、4.5 g/(kg·d),妈咪爱组灌胃妈咪爱0.2 g/(kg·d),连续14 d.给药结束后,脱颈处死大鼠,免疫组化法测定大鼠血清中VIP、CCK的表达.结果:与空白对照组比较,模型组脑肠肽VIP、CCK表达显著增加(P<0.05).与模型组比较,妈咪爱组脑肠肽VIP、CCK表达降低,健胃止泻合剂低、高剂量组脑肠肽VIP表达显著降低(P<0.05),健胃止泻合剂低、高剂量组脑肠肽CCK表达显著降低(P<0.05).结论:健胃止泻合剂可通过降低脾虚型功能性腹泻大鼠脑肠肽VIP、CCK的表达,调节肠道运动和水液代谢,从而改善功能性腹泻.

Abstract

Objective:To observe the effects of different doses of Jianwei Zhixie Mixture on the expression of cerebral intestinal peptide VIP and CCK in rats with functional diarrhea with spleen deficiency.Methods:50 SPF rats were randomly divided into blank control group,model group,Mommy love group,Jianwei Zhixie Mixture low-dose group and Jianwei Zhixie Mixture high-dose group with 10 rats each.The Mamai group was given 0.2g/(kg·d)intragastric administration,and the low and high dose Jianwei Zhixie Mixture groups were given 4.5 and 18g/(kg·d)Jianwei Zhixie Mixture intragastric administration,respectively,for a total of 14 days.The rats in the model group and the blank control group were given 0.9%NaCl solution freely every day for 14 days.After administration,the rats were killed by neck removal,and the expressions of VIP and CCK in serum were determined by immunohistochemistry.Results:Compared with blank control group,the expressions of VIP and CCK were significantly increased in model group(P<0.05).Compared with the model group,the expression of cerebral intestinal peptide VIP and CCK decreased in the mommy love group.Compared with model group,VIP expression of brain-gut peptide was significantly decreased in low-dose and high-dose Jianwei Zhixie Mixture groups(P<0.05),CCK expression of brain-gut peptide was significantly decreased in high-dose Jianwei Zhixie Mixture group(P<0.05).Conclusion:Jianwei Zhixie Mixture can improve functional diarrhea by reducing the expression of VIP and CCK,regulating intestinal movement and water metabolism in rats with functional diarrhea of spleen deficiency.

关键词

健胃止泻合剂/脾虚型功能性腹泻/VIP/CCK

Key words

Jianwei Zhixie Mixture/Functional diarrhea with spleen deficiency/VIP/CCK

引用本文复制引用

出版年

2024
中医药信息
中华中医药学会,黑龙江中医药大学

中医药信息

影响因子：1.219
ISSN：1002-2406
段落导航相关论文