In order to ensure the historical value of paper cultural relics,the application research of biological enzymes in the removal of mold spots on paper cultural relics is proposed. The proposed method aims to identify the types of fungi on the surface of ancient book paper pages to be processed. After wiping and sampling the paper cultural relics samples,the DNA of the strains is extracted using the rapid micro extraction method,and the OTU clustering method is used to identify the types of fungi,laying a foundation for the selection of biological enzymes in subsequent cleaning reagents. After completing the selection of targeted antifungal enzyme agents and matching surfactants,a siphon cleaning device was used to test the optimal ratio of cleaning agents on the surface of simulated samples with mold spots. Through testing the tensile strength change and color difference change of sample paper under different AOS surfactant addition concentrations,the optimal concentration of surfactants was determined to further optimize the antifungal effect of the enzyme complex cleaning agent.