首页|南疆沙漠环境下三种绵羊的抗逆性基因研究

南疆沙漠环境下三种绵羊的抗逆性基因研究

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基于全基因组高密度SNP芯片技术,针对策勒黑羊、和田羊和多浪羊进行耳组织采集、DNA提取、基因组芯片制作等,通过核苷酸多样性指数PI法进行基因组选择性清扫,分析寻找三种绵羊品种抗逆性相关基因.研究发现,共获沙漠环境抗逆性相关的候选基因有 1 057 个,对其进行GO和KEGG分析,并利用STRING进行蛋白网络分析,发现具有抗逆性相关的基因有64 个,例如,IL20Rβ、LEPR、SCG2、PTPRO、KLF5、KLF12 等.通过基因组选择性清扫技术在南疆绵羊品种中寻找到 1 058 个相关环境适应性的候选基因,为南疆绵羊的引种和改良提供参考,并为解析南疆绵羊品种在沙漠环境下抗逆性机制提供参考.
Study on stress resistance genes of three kinds of sheep in desert environment of southern Xinjiang
Based on the high-density SNP chip technology of the whole genome,ear tissue collection,DNA extraction,and genome chip production were carried out for Zele black sheep,Hetian sheep,and Duolang sheep.Genomic selective sweeping was performed using the nucleotide diversity index(PI)method to analyze and search for stress resistance related genes in the three sheep breeds.A study found that a total of 1057 candidate genes related to desert environment stress resistance were obtained,and GO and KEGG analyses were conducted on them.Protein network analysis was performed using online software STRING,and 64 genes related to stress resistance were found,such as IL20R β,LEPR,SCG2,PTPRO,KLF5,KLF12,etc.Through genome selective sweeping technology,1058 candidate genes related to environmental adaptability were identified in southern Xinjiang sheep breeds,providing reference for the introduction and improvement of southern Xinjiang sheep,and providing reference for analyzing the stress resistance mechanism of southern Xinjiang sheep breeds in desert environments.

Sheepresistance to adversitydesert environment in southern Xinjiangselective cleaningPI value of nucleotide polymorphism

邢子豪、孔令锋、彭玉薇、李子翾、芮雪、王佳旭、王金龙、杨建礼、马正委、王权锋、刘书东

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塔里木大学,新疆 阿拉尔 843300

农业农村部环塔里木畜草资源利用重点实验室,新疆 阿拉尔 843300

和田地区津垦牧业科技有限公司,新疆 和田 848400

绵羊 抗逆性 南疆沙漠环境 选择性清扫 核苷酸多态性PI值

2024

现代牧业
郑州牧业工程高等专科学校

现代牧业

影响因子:0.137
ISSN:1008-3111
年,卷(期):2024.8(3)