采用常规形态学原理，结合核糖体 DNA 转录间隔区（ITS）序列分析技术，对我国4个羊肚菌主要栽培菌株和5个主要采集自川渝地区的野生分离物进行菌株鉴定和系统发育分析，研究遵循羊肚菌 MLST 数据库的序列鉴定程序，基于系统研究过的可靠序列信息，对获得的 ITS 序列进行逐一比对，选取相似度靠前的信息，构建系统发育树。结果表明，目前我国大面积种植的羊肚菌分别为梯纹羊肚菌、六妹羊肚菌和七妹羊肚菌；5个野生种质分别鉴定为梯纹羊肚菌和高羊肚菌。
Strain identification and phylogenetic analysis of cultivated and wild strains of Morchella belonging to Elata Clade in China
Applying regular morphology combined with the technology of ITS sequence analysis,the identi-fication and phylogenetic analysis of the 4 cultivated strains and 5 wild morel isolates mainly collected from Sichuan province and Chongqing municipality were carried out.The study followed the sequence identifica-tion program required by data bank of multilocus sequence typing(MLST).The phylogenetic tree was con-structed with reliable sequences obtained from BLAST one-by-one comparison.The results suggested that the cultivated morel varieties were identified as Morchella importuna,M.sextelata and M.septimelata.In addition,5 wild isolates were identified as M.elata and M.importuna,respectively.
国家科技期刊平台
NSTL
万方数据
维普
