The effect of mold on the fungal community structure during the industrial fermentation of cigar tobacco leaves
To investigate the changes in fungal community structure with mold occurrence during the industrial fer-mentation of cigar tobacco leaves,Dexue No.1 cigar tobacco leaf was chosen as the test material,and counting methods in microbiology and Illumina MiSeq high-throughput sequencing technology were used to analyze the chan-ges in mold content and fungal community structure of normal and moldy cigar tobacco leaf samples during the industrial fermentation.The results indicated that compared to norml tobacco leaves,on the 30th day of fermenta-tion,the mold content in moldy cigar tobacco leaves was the highest(8.3×104 CFU/g),the richness and diversity of species were the lowest,and the number of OTUs was the least.At the level of the phylum,Ascomycota was an absolute advantageous phylum on day 30 of fermentation,with an average relative abundance of 99.82%.At the level of the genus,Aspergillus was the main genus in the entire fermentation process,and the average relative abun-dance on day 30 of fermentation was the largest(92.32%).The 30 d mildewy cigar tobacco leaf and other samples had a significant difference in species composition compared to normal tobacco leaves.Among them,Aspergillus was both an advantageous genus and a differential genus,and the average relative abundance of various samples was the highest.
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