Research on extraction process of carotenoids from yeasts and algae based on ionic liquid
Using Rhodotorula glutinis and Chlorella vularis sludge as raw material,the extraction process of carote-noids from yeast and algae was optimized through single factor and response experiments,and the in vitro antioxi-dant activity of carotenoids from yeast and algae was investigated.The results showed that the optimal ionic liquid for yeast and algal wall breaking was 1-butyl-3-methylimidazole chloride(BMIMCl),and the optimal extraction process for carotene from yeast and algal sludge was 1 mol/L ionic liquid concentration,pre-treatment temperature of 53℃,and pre-treatment time of 57 min.Under this condition,the extraction amount of carotene from yeast and algae was 1.311 mg/g.When the mass concentration was 30 μg/mL,the scavenging rates of DPPH free radical,·OH free radical and ABTS+ free radical were 82.3%,37.6%and 86.8%,respectively,and the reducing power was 0.289,showing good antioxidant activity.The yeast and algal sludge treated with BMIMCl had a higher content of extracted carotenoids and better antioxidant activity.
ionic liquidyeast and algal sludgecarotenoidsextraction processantioxidant activity
国家科技期刊平台
NSTL
万方数据
维普
