Effect of Eucommia ulmoides Oliv.leaf polysaccharide on bile salt tolerance of Lactobacillus plantarum CICC 20022
With Lactobacillus plantarum CICC 20022 as the research object,this study investigated the effects of Eucommia ulmoides leaf polysaccharides on the number of viable bacteria,microstructure,fatty acid composition of cell membrane,and structural integrity of cell membrane and cell wall under bile salt stress.The results showed that the viable count of L.plantarum CICC 20022 was significantly increased(P<0.05),when the crude polysac-charide or the refined polysaccharide of E.ulmoides leaf were added to the bile salt stress environment and cultured for 24 h,which was 1.30 times or 1.10 times that of the control,respectively.Compared with the control,the addition of crude polysaccharide from E.ulmoides leaf or refined polysaccharide from E.ulmoides leaf could signifi-cantly protect the microstructure of bacteria and reduce the cell damage caused by bile salt stress,and the PI fluo-rescence intensity,alkaline phosphatase(AKP)activity and DNA leakage of cells were significantly reduced(P<0.05),while the ratio of unsaturated fatty acids to saturated fatty acids(U/S)in cell membrane was significantly increased(P<0.05)to 0.37.The results indicated that adding crude polysaccharide from E.ulmoides leaf in the environment of bile salt stress could maintain the integrity of cell structure of L.plantarum CICC 20022 and improve its bile salt tolerance.
Eucommia ulmoides Oliv.leaf polysaccharideLactobacillus plantarum CICC 20022bile salt tolerance
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