首页|5种火炬花花朵形态特征及柱头可授性检测

5种火炬花花朵形态特征及柱头可授性检测

Morphological characteristics and fertility detection of five Kniphofia uvaria varieties

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以'木瓜棒冰''芒果棒冰''燃烧''波科橙色'和'波科黄色'5种火炬花为材料,比较花朵形态特征、花粉活力和柱头可授性.采用感官测量法测量计算花朵形态特征、醋酸洋红染色法、不同蔗糖浓度进行花粉离体培养检测花粉活力,联苯胺—过氧化氢法测定柱头可授性,并进行人工授粉.结果表明5个品种火炬花颜色由浅黄色至橙红色,'木瓜棒冰'花形花色表现最好,最具观赏性;花粉活力最高的'波科橙色'为69.18%;花粉活力最低的'芒果棒冰'为36.42%.花粉离体萌发率表现较好的是5%蔗糖浓度下,'燃烧'萌发率为2.08%;10%蔗糖浓度下,'波科橙色'的萌发率为2.38%.'木瓜棒冰'的柱头可授性最高,'波科橙色'和'波科黄色'的柱头可授性极低.人工授粉3个组合中,'木瓜棒冰'×'芒果棒冰'的结果率最高,约为40%.本研究为火炬花的人工杂交育种提供了实验依据.
'Papaya Popsicle''Mango Popsicle''Fire Glow''Poco Orange'and'Poco Yellow'were used to compare flower morphological characteristics and investigate the pollen viability and stigma receptivity.Morphological characteristics of flowers were observed by sensory measurements.The pollen viability was assessed using the aceto-carmine staining method and compared by in vitro cul-ture with different sucrose concentration.The stigma receptivity was detected by benzidine-H2O2 per-oxide method and artificial pollination.The results showed that the flower shape and color of'Papa-ya Popsicle'were the best.The highest and the lowest of the pollen viability were'Poco Orange'(69.18%)and'Mango Popsicle'(36.42%)respectively.The pollen germination results showed that a 5%sucrose concentration was more suitable for'Fire Glow'in vitro culture with a germination rate of 2.08%,a 10%sucrose concentration was more suitable for'Poco Orange'in vitro culture with a germination rate of 2.38%.The results of stigma receptivity showed that the receptivity of'Papaya Popsicle'was the best,'Poco Orange'and'Poco Yellow'were extremely low.The highest fruit set-ting rate was 40%of'Papaya Popsicle'×'Mango Popsicle'among the three hybridization combina-tions.The study provides an experimental basis for the artificial hybrid breeding of Kniphofia uvaria.

Kniphofia uvariapollen viabilitypollen germinationstigma receptivityartificial polli-nation

王玉玲、覃茜倪、余惠文、陆銮眉

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闽南师范大学生物科学与技术学院,福建 漳州 363000

闽南师范大学闽台特色园林植物福建省高校重点实验室,福建 漳州 363000

火炬花 花粉活力 花粉萌发 柱头可授性 人工授粉

2024

闽南师范大学学报(自然科学版)
漳州师范学院

闽南师范大学学报(自然科学版)

影响因子:0.272
ISSN:1008-7826
年,卷(期):2024.37(4)