首页|Novel authentication approach for coffee beans and the brewed beverage using a nuclear-based species-specific marker coupled with high resolution melting analysis
Novel authentication approach for coffee beans and the brewed beverage using a nuclear-based species-specific marker coupled with high resolution melting analysis
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NSTL
Elsevier
Coffee is a very popular and highly traded commodity that is targeted by fraudulent practices, affecting global production and economy. A common practice involves the substitution of the more expensive Arabica coffee with the less popular Robusta. Various physiochemical and DNA-based technologies have been used for coffee authentication; yet detection of adulterants in coffee still faces limitations in terms of the raw material used, the level of processing, and the sensitivity of available analytical tools. Additionally, DNA-based methodologies cannot be successfully used in the presence of strong inhibitors in brewed coffee. Herein, we designed an Internal Transcribed Spacer region 2 (ITS2)-based marker that allowed coffee authentication, even from the brewed beverage, when coupled with High Resolution Melting (HRM) analysis. The developed methodology could effectively detect differences in Arabica/Robusta admixtures, as low as 1% (v/v) DNA content. Direct species identification from the brewed beverage was enabled by the use of an inhibitor-tolerant DNA polymerase with enhanced sensitivity, without prior DNA purification. In the context of food safety, this novel approach could advance coffee authentication methods for safeguarding coffee producers and consumers.
NSTL
Elsevier
