首页|Simultaneous Determination of Five Components Including Acetaminophen by Reversed-phase High Performance Liquid Chromatography

Simultaneous Determination of Five Components Including Acetaminophen by Reversed-phase High Performance Liquid Chromatography

扫码查看
原文链接
  • NETL
  • NSTL
High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup, including acetaminophen, p-aminophenol, caffeine, chlorphenamine maleate and guaifenesin. The mobile phase consists of 15 wt percent acetonitrile, 0.004 mol/L sodium heptyl sulfonate, 0.03 mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13:40:44:3), the pH of which is adjusted to 3.0 by phosphoric acid. The contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm, with a flow rate of 0.4 mL/min. The results show that the calibration curves are linear in a certain range. The average recovery of five components is between 96.31 percent and 102.3 percent.

organic compoundspreparation and determinationchromatography

ZHANG Li-qing、WU Xiao-hua、LU Ying

展开 >

Zhejiang University of Science and Technology

2004

Journal of Wuhan University of Technology: Materials science edition

Journal of Wuhan University of Technology: Materials science edition

ISSN：1000-2413
年,卷(期)：2004.19(1)