首页|Protective Effect of Carvedilol on Abnormality of L-type Calcium Current Induced by Oxygen Free Radical in Cardiomyocytes
Protective Effect of Carvedilol on Abnormality of L-type Calcium Current Induced by Oxygen Free Radical in Cardiomyocytes
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The protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in single guinea pig ventricular myocytes was studied. Whole-cell patch clamp technique was used to study the effect of H_2O_2 (0. 5 mmol/L) on L-type calcium current in single guinea pig ventricular myocytes and the action of pretreatment with carvedilol (0. 5 μmol/L). 0. 5 μmol/L carvedilol had no significant effect on I_(Ca,L) and its channel dynamics. In the presence of 0. 5 mmol/L H_2O_2, peak current of I_(Ca,L) was reduced significantly (P < 0. 001), the Ⅰ-Ⅴ curve of I_(Ca,L) was shifted upward, steady-state activation curve and steady-state deactivation curve of I_(Ca,L) were shifted left and recovery time of I_(Ca,L) was delayed significantly (P < 0. 001). 0. 5 μmol/L carvedilol significantly alleviated the inhibitory effect of H_2O_2 on I_(Ca,L) as compared with that in H_2O_2 group (P < 0. 01). In addition, carvedilol reversed the changes of dynamics of I_(Ca,L) induced by H_2 O_2. It was concluded that carvedilol could alleviate the abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes. It shows partly the possible mechanism of the special availability of carvedilol in chronic heart failure.
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