首页|Establishment and identification of fibroblast clones expressing human bone morphogenetic protein 2
Establishment and identification of fibroblast clones expressing human bone morphogenetic protein 2
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Objective: To establish fibroblasts stably expressing human bone morphogenetic protein 2( hBMP2). Methods: Eukaryonic expression vector(pcDNA3.1-B2) was transduced into NIH3T3 cells using Sofast~(TM), a new generation cationic polymer gene transfection reagent. The positive cell clones were selected with G418. The stable transfection and expression of BMP2 in the NIH3T3 cells were determined by RT-PCR and immunohistochemical stain. Results; BMP2 mRNA was transcripted and expressed in the transfected NIH3T3 cells. Conclusion; With positive compound transfection, outside human BMP2 gene can be successfully transducted into NIH3T3 cells, which is the key step to induce periodontal cells to osseous phenotypes.
bone morphogenetic protein 2gene transfectionfibroblastic cells
Juan Wang、Weibin Sun、Chun Lu、Guixia Tang
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Stomatological College, Nanjing Medical University, Nanjing 210029, China
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NSTL
