首页|Cre/lox-mediated Marker Gene Excision in Elite Indica Rice Plants Transformed with Genes Conferring Resistance to Lepidopteran Insects
Cre/lox-mediated Marker Gene Excision in Elite Indica Rice Plants Transformed with Genes Conferring Resistance to Lepidopteran Insects
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
Cre/lox-mediated gene excision in commercial rice (Oryza sativa L.) plants was studied with a recombination-reporter gene system, in which the selectable marker hygromycin phosphotransferase gene (hpt) flanking by two directly oriented lox sites was located between the rice actin1 promoter and a promoterless gusA gene. This system allows visualization of GUS expression by activating promoterless gusA after site-specific recombination. The crossing strategy was used to introduce the cre gene into the hx plants. In 30 hybrid plants from four crosses made from T_0 actin1 promoter-lox-hpt-lox -gusA plant with T_0 cre plant, 12 expressed GUS and 9 showed hygromycin-sensitive. We furthermore demonstrated the utility of the Cre/lox in excision of hpt marker gene in an elite indica rice restorer Minghui 86 transformed with both insecticidal modified cowpea trypsin inhibitor gene sck and Bacillus thuringi'ensis endotoxin gene cryIAc, In 77 hybrid plants from nine crosses made from T_2 homozygous lox-hpt-lox-sck-cryIAc plant with T_2 homozygous cre plant, 56 showed hygromycin-sensitive. Molecular analyses confirmed the excision of hpt in all hygromycin-sensitive plants.
NETL
NSTL
