首页|COMPARISON OF DIFFERENT STAINING METHODS FOR ANALYSING ESTERASE (EST) ISOZYME OF FUNGI AND PLANTS
COMPARISON OF DIFFERENT STAINING METHODS FOR ANALYSING ESTERASE (EST) ISOZYME OF FUNGI AND PLANTS
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EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi, and the staining is an important process in electrophoresis analysis of studying Est. Be effects were compared among different stain recipes for Est of 3 kinds of fungi-Lentinus edodes, Pleurotus sapidus, Phellinus igriarius and 2 kinds of platits-Populus sp and Brassica chinensts. Of the four kinds of Est staining recipes tested, the recipe alpha-acetic acid-naphther showed the best effect. and followed bybeta-aceticacid-naphther, semicontent alpha-aceticacid-naphthet and alpha+beta-acetic acid-
FungiEsterase isozymeStaining methods
Chi Yujie、Zhang Ming、Dong sheng、Xu Cuiqing
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Northeast Forestrt University. 150040 Harbin, China
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