首页|Bone Formation and Healing Study of hBMP-2 Transfected rMSCs Attached to DBM
Bone Formation and Healing Study of hBMP-2 Transfected rMSCs Attached to DBM
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Objective: To detect the effect of hBMP-2 transfected rMSCs on bone repair and the capability of the new biomaterial in enhancing bone repair. Methods: Auto-rMSCs were cultured and transfected with hBMP-2 by liposome. All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new bio-materials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods. Results: The osteoblasts could be seen in the biomaterials with transfected rMSCs, which have been implanted in muscle bags. There was no sign of bone formation in the biomaterials with untransfected rMSCs and the single DBM groups. With the segmental bone defects, all the transfected, untransfected and single DBM biomaterials could work, but the healing of the biomaterial with transfected hBMP-2 was the fastest and most effective. Conclusion: Delivery of rMSCs with transfected hBMP-2 genemay generate osteo-morphogenesis and promote skeletal repair in vivo.
human bone morphogenetic protein-2rabbit mesenchymal stem cellsdemineralized bone matrixbone repair
LI Jun、FAN Qing-yu、YU Lan、FAN De-gang、WEN Yan-hua
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Department of Orthopaedic Surgery, Tangdu Hospital, the Forth Military Medical University, Xi' an 710038, P. R. China
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