Therapeutic cloning refers to the derivation of embryonic stem cells (ntESC) from embryos derived from somatic cell nuclear transfer (SCNT) also known as cloning. Cloning involves transplanting a differentiated cell into an oocyte that has had its nucleus (DNA) removed. The reconstructed oocyte can be activated to divide and develop into an embryo. The process that allows this to happen is termed nuclear reprogramming, and is defined as the mechanism through which a differentiated cell de-differentiates or returns to a totipotent state (capable of giving rise to any cell type, including extra-embryonic) and directs embryonic development. Cells from blastocyst stage cloned embryos can be used to generate ntESC lines.
Xiangzhong Yang、Sadie L Smith
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Center for Regenerative Biology and Department of Animal Science, University of Connecticut, Storrs, CT06269, USA
NETL
NSTL
