首页|PHA production from the organic fraction of municipal solid waste (OFMSW): Overcoming the inhibitory matrix
PHA production from the organic fraction of municipal solid waste (OFMSW): Overcoming the inhibitory matrix
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NETL
NSTL
Elsevier
Leachate from the source separated organic fraction of municipal solid waste (OFMSW) was evaluated as a substrate for polyhydroxyalkanoates (PHA) production. Initially, the enrichment step was conducted directly on leachate in a feast-famine regime. Maximization of the cellular PHA content of the enriched biomass yielded to low PHA content (29 wt%), suggesting that the selection for PHA-producers was unsuccessful. When the substrate for the enrichment was switched to a synthetic volatile fatty acid (VFA) mixture-resembling the VFA carbon composition of the leachate-the PHA-producers gained the competitive advantage and dominated. Subsequent accumulation with leachate in nutrient excess conditions resulted in a maximum PHA content of 78 wt%. Based on the experimental results, enriching a PHA-producing community in a "clean" VFA stream, and then accumulating PHA from a stream that does not allow for enrichment but does enable a high cellular PHA content, such as OFMSW leachate, makes the overall process much more economically attractive. The estimated overall process yield can be increased four-fold, in comparison to direct use of the complex matrix for both enrichment and accumulation. (C) 2016 Elsevier Ltd. All rights reserved.
Wastewater treatmentOrganic fraction of municipal solid waste (OFMSW)LeachatePolyhydroxyalkanoates (PHA)Mixed microbial enrichmentsFeast-famine
Korkakaki, Emmanouela、Mulders, Michel、Veeken, Adrie、Rozendal, Rene、van Loosdrecht, Mark C. M.、Kleerebezem, Robbert
NETL
NSTL
Elsevier
