首页|Structure-activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations
Structure-activity relationships of the ATP cofactor in ligase-catalysed oligonucleotide polymerisations
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NETL
NSTL
Royal Soc Chemistry
A T4 DNA ligase-catalysed oligonucleotide polymerisation process has been recently developed to enable the incorporation of multiple functional groups throughout a nucleic acid polymer. T4 DNA ligase requires ATP as a cofactor to catalyse phosphodiester bond formation during the polymerisation process. Herein, we describe the structure-activity relationship of ATP within the context of T4 DNA ligase-catalyzed oligonucleotide polymerisation. Using high-throughput sequencing, we study not only the influence of ATP modification on polymerisation efficiency, but also on the fidelity and sequence bias of the polymerisation process.
Yi Lei、Ryan Hili
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Department of Chemistry, University of Georgia, Athens, GA 30602, USA
NETL
NSTL
Royal Soc Chemistry
