首页|Investigation on polyphosphate accumulation in the sulfur transformation-centric EBPR (SEBPR) process for treatment of high- temperature saline wastewater

Investigation on polyphosphate accumulation in the sulfur transformation-centric EBPR (SEBPR) process for treatment of high- temperature saline wastewater

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  • Elsevier
This study investigated the polyphosphates accumulation rate in a novel sulfur transformation-centric enhanced biological phosphorus removal (SEBPR) process. The SEBPR system was continuously operated over 120 days in a sequencing batch reactor (SBR) that alternated between the anaerobic mode and the anoxic mode of operation (temperature: 30 degrees C and salinity: 6000 mg/L Cl-). In addition to the SBR, batch experiments were carried out to test the effect of two different sulfate concentrations on the system performance and sulfur-phosphorus transformations. The key intercellular polymers of polyphosphates and polysulfur (poly-S) were identified by employing advanced microscopes. Metagenomic analysis was performed to characterize the diversity of microbes and their functions enriched in the SEBPR system. Finally, several molecular techniques including flow cytometry cell sorting and 16S DNA high-throughput sequencing were applied to identify the phosphorus-accumulating organisms (PAOs). The amounts of P release and P uptake in the SEBPR increased gradually to nearly 18 +/- 6.4 mg P/L, and 26.5 +/- 6.7 mg P/L respectively, yielding a net P removal efficiency of 84 +/- 25%. Batch tests indicated no polyhydroxyalkanate (PHA) synthesis, but P uptake was observed and it was correlated with the intracellular poly-S consumption, suggesting that the poly-S could act as an intracellular energy source for P uptake and polyphosphates formation. Moreover, CLSM and TEM micrographs clearly showed the presence of intercellular polyphosphates and poly-S respectively. Metagenomic analysis revealed that Proteobacteria (36.5%), Bacteroidetes (23.3%), Thermotogae (7.1%), Chloroflexi (4.5%) and Firmicutes (2.3%) were the dominant phyla in Bacteria. The conventional PAO of Candidatus Accumulibacter was found at a low abundance of 0.32% only; and an uncultured genus close to Rhodobacteraceae at the family level is speculated to be the putative sulfur PAO (SPAO). Finally, this research suggests that poly-S considerably impacts on polyphosphates accumulation in the SEBPR system when no PHAs are formed. (C) 2019 Elsevier Ltd. All rights reserved.

Saline wastewaterSulfur transformation-centric EBPR (SEBPR)PolyphosphatesPoly-S Phosphorus-accumulating organisms (PAOs)Sulfur bacteria

Wang, Hai-Guang、Huang, Hao、Liu, Ru-Long、Mao, Yan-Ping、Biswal, Basanta Kumar、Chen, Guang-Hao、Wu, Di

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Hong Kong Univ Sci & Technol, Dept Civil & Environm Engn, Chinese Natl Engn Res Ctr Control & Treatment Hea, Hong Kong Branch, Hong Kong, Peoples R China|Hong Kong Univ Sci & Technol, Water Technol Ctr, Hong Kong, Peoples R China

Shanghai Ocean Univ, Dept Marine Sci, Shanghai, Peoples R China

Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen, Guangdong, Peoples R China

Hong Kong Univ Sci & Technol, Dept Civil & Environm Engn, Chinese Natl Engn Res Ctr Control & Treatment Hea, Hong Kong Branch, Hong Kong, Peoples R China|Hong Kong Univ Sci & Technol, Water Technol Ctr, Hong Kong, Peoples R China|Hong Kong Univ Sci & Technol, FYT Grad Sch, HKUST Shenzhen Res Inst, Shenzhen, Guangdong, Peoples R China

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2019

10.1016/j.watres.2019.115138

Water research: A journal of the international water association

Water research: A journal of the international water association

EI
ISSN：0043-1354
年,卷(期)：2019.167(Dec.15)
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