首页|Effectiveness of different sources of nitrogen in MS media for rapid in vitro shoot initiation of sugarcane (Saccharum officinarum L.) genotypes
Effectiveness of different sources of nitrogen in MS media for rapid in vitro shoot initiation of sugarcane (Saccharum officinarum L.) genotypes
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Tissue culture techniques are important tools of crop improvement and rapid multiplication of sugarcane clones. Nitrogen is essential not only for growth, but also for morphogenesis in tissue culture, which is markedly influenced by the availability of nitrogen and the form in which it is presented in the plant. In a laboratory experiment with sugarcane genotypes Co 86032, CoVC 15-22-05, CoVC 15-22-26 and CoVC 18061, the efficacy of different sources of nitrogen; namely ammonium sulphate, tri ammonium citrate and supplement for MS (Murashige and Skoog) medium were evaluated. There was no consistent response from genotypes from the different N sources in the medium. The results indicated that the interaction effect of sugarcane genotypes and different nitrogen sources was found significant for various shoot initiation and shoot induction characteristics. Findings showed that the genotype Co 86032 performed best in the medium fortified with supplement for MS for various growth parameters viz., days for explant to turn autotrophic, autotrophic frequency, days for appearance of axillary buds, axillary shoots per explant, length of axillary shoots, days of shoot establishment, shoot length after establishment and shoot induction frequency. Genotypes, CoVC 15-22-26 and CoVC 15-22-05 showed optimum response in the MS medium supplemented with ammonium sulphate, while genotype, CoVC 18061, performed best when MS medium was supplemented with tri-Ammonium citrate.
sugarcanetissue cultureMS Mediumnitrogen
Yadav, Suresh、Nagaraja, T. E.、Lohithaswa, H. C.、Shivakumar, K. V.、Taku, Meniari
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Indian Agr Res Inst, Div Genet, New Delhi 110012, India|Univ Agr Sci, Bengaluru, India