首页|Efficacy of a typical clean-in-place protocol against in vitro membrane biofilms
Efficacy of a typical clean-in-place protocol against in vitro membrane biofilms
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NETL
NSTL
Elsevier
This study evaluates the effectiveness of a typicalclean-in-place (CIP) protocol against in vitro biofilmson whey reverse osmosis (RO) membranes developedunder static condition。 Bacterial isolates obtained fromRO membrane biofilms were used to develop singleand multispecies biofilms under laboratory conditions。A typical commercial CIP protocol was tested againstthe 24-h-old biofilms， and included 6 sequential treatmentsteps based on alkali， surfactant， acid， enzyme，a second surfactant， and a sanitizer treatment step。Experiments were conducted in 4 replicates and thedata were statistically analyzed。 The results revealeda variation in the resistance of mixed-species biofilmsagainst the individual steps in the sequential CIP protocol。The overall 6 steps protocol， although resultedin a greater reduction， also resulted in the detectionof survivors even after the final sanitizer step， reflectthe ineffectiveness of the CIP protocol for completeremoval of biofilms。 Posttreatment counts of 0。71 logafter the sequential CIP of mixed-species biofilm revealedthe resistance of biofilm constitutive microbiota。Mixed-species biofilms， constituting different generaincluding Bacillus， Staphylococcus， and Streptococcus，were observed to be more resistant than most of thesingle-species biofilms。 However， among the single-speciesbiofilms， significantly different resistance patternwas observed for Bacillus isolates compared with theother bacterial isolates。 All 5 isolates of Bacillus werefound resistant with survivor counts of more than 1。0log against the sequential CIP protocol tested。 Thus， itcan be concluded that the tested CIP protocol had alimited effectiveness to clean membrane biofilms formedon the whey RO membranes。
biofilmswheyreverse osmosisCIP
Diwakar Singh、Sanjeev Anand
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Midwest Dairy Foods Research Center, Dairy and Food Science Department, South Dakota State University, Brookings 57006
NETL
NSTL
Elsevier
