首页|Enhancement of biological effects of oxidised nano- and microplastics in human professional phagocytes

Enhancement of biological effects of oxidised nano- and microplastics in human professional phagocytes

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  • NETL
  • NSTL
  • Elsevier
Micro and nanoplastics are ubiquitous pollutants that can cause adverse health effects even in humans. Effects of virgin and oxidised (simulating the aging processes) polystyrene nano (nPS) and micro particles (mPS) with diameters of 0.1 and 1 μm were studied on human professional phagocytes (i.e., monocyte cells THP-1 and macrophage-like mTHP-1 cells). After characterization by ATR-FTIR, UV-Vis spectroscopy, SEM and dynamic light-scattering analyses, the particles were FITC functionalised to quantify cellular uptake. Changes in the cell compartments were studied by acrydine orange and the pro-oxidant, cytotoxic and genotoxic effects were assessed. Phagocytosis was dose- and time- dependent and at 24 h 52% of nPS and 58% of mPS were engulfed. Despite the high homeostasis of professional phagocytes, significant ROS increases and DNA damage were observed after exposure to oxidised particles. The results highlight that the environmental aging processes enhances the adverse health effects of micro and nanoplastics.

Nano and micro polystyrene particleUptakeHuman monocyteMacrophage-like cellsEnvironmental wear processesPro-oxidant effects

Giuseppa Visalli、Antonio Lagana、Alessio Facciola、Antonella Iaconis、Jessica Curcio、Santa Pollino、Consuelo Celesti、Silvia Scalese、Sebania Libertino、Daniela Iannazzo、Angela Di Pietro

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Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, Italy

Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, Italy,Istituto Clinico Polispecialistico COT. Cure Ortopediche Traumatologiche s.p.a., 98124 Messina, Italy

Department of Electronic Engineering, Industrial Chemistry and Engineering, University of Messina, 98125 Messina, Italy

Istituto per la Microelettronica e Microsistemi, Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada n.5,1-95121 Catania, Italy

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2023

10.1016/j.etap.2023.104086

Environmental toxicology and pharmacology

Environmental toxicology and pharmacology

SCI
ISSN：1382-6689
年,卷(期)：2023.99(Apr.)
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