首页|A pipette-smartphone portable on-site detection system for aflatoxin B_1 detection in bread and peanut based on 'Four-in-one' sea-urchin-like composite catalyst

A pipette-smartphone portable on-site detection system for aflatoxin B_1 detection in bread and peanut based on 'Four-in-one' sea-urchin-like composite catalyst

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  • NETL
  • NSTL
  • Elsevier
The development of a low-cost, high-sensitivity, portable bioassay for detecting the highly toxic and thermally stable aflatoxin B_1(AFB_1) is of significant importance to food safety and human health. A pipette-smartphone integrated biosensor based on a super "four-in-one" DNA sea-urchin-like composite catalyst (FSCC) was constructed for the efficient analysis of AFB_1. Detection is possible via a self-made poly(methyl methacrylate) pipette, combined with a smartphone to further increase the biosensor's portability. The combination of the two greatly simplifies experimental operations, reduces testing costs, and can minimize matrix interferences through sample pre-treatment operations. And the smartphone-APP signal readout strategy can avoid the need for a reading instrument, making it more suitable for field testing. The FSCC was developed by fully utilizing the properties of a Fe-Zr-metal-organic framework (Fe-Zr-MOF), which exhibits high catalytic activity and enables the encapsulation of abundant horseradish peroxidase in its pores, the in-situ reduction and enrichment of Pt nanoparticles on its surface, and the coordination of biotinylated DNA to achieve a high adsorption capacity of streptavidin-horseradish peroxidase. This ensured detection sensitivity, with a limit of detection = 1.57 pg/mL. The developed biosensor displays notable application potential, especially in resource-poor areas due to the advantages of low costs, simple operation, ultra-high sensitivity, portability, etc.

Food safetyOn-site detectionComposite catalystColorimetric immunoassay

Feng Hong、Liangqiong Ren、Feng Jiang、Shilong Ruan、Jianhan Lin、Jianlong Wang、Xiaobo Hu、Yiping Chen

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College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan 430075, China

Daye Public Inspection and Test Center, Daye 435100, Hubei, China

Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shanxi, China

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China, Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan 430075, China

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2023

10.1016/j.snb.2023.134587

Sensors and actuators, B. Chemical

Sensors and actuators, B. Chemical

ISSN：0925-4005
年,卷(期)：2023.397(Dec.)
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