首页|Antibody binding to plasmonic nanostructure: A validation study using experiment and simulation

Antibody binding to plasmonic nanostructure: A validation study using experiment and simulation

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Localized surface plasmon resonance (LSPR) sensors allow the label-free detection of molecules in real-time with a high sensitivity, which makes them suitable for the detection of various molecules. However, the experimental characterization of the surface functionalization and the molecular binding on the nanostructured sensor surface represent black boxes. To overcome these uncertainties, this study shows an approach to simulate and experimentally validate the plasmonic response upon molecular binding to a LSPR sensor for the detection of diclofenac (DCF). Assuming homogeneous molecular coverage and averaged parameters such as refractive index and layer thickness through the combination of an iterative experimental validation and electrodynamics simulations a coherent model was formed that includes the uncertainties of molecular binding. This validated model and the obtained parameter for the biochemical assay forms the basis for a future automated optimization of the nanostructure geometry with respect to increased sensitivity to molecular binding.

FDTD simulationLocalized surface plasmon resonance (LSPR)BiosensingMolecular bindingNanostructure

Kevin Thomschke、Nadja Steinke、Marisa Rio、Thomas Hartling、Christiane Schuster

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Fraunhofer Institute for Ceramic Technologies & Systems IKTS, Maria-Reiche-Str. 2, Dresden 01109, Germany

Fraunhofer Portugal Center for Smart Agriculture and Water Management AWAM, Parque de Ciencia e Tecnologia de Vila Real - Regia Douro Park, Vila Real 5000-033, Portugal

Fraunhofer Institute for Ceramic Technologies & Systems IKTS, Maria-Reiche-Str. 2, Dresden 01109, Germany, Technische Universitaet Dresden, Institute of Solid-State Electronics, Mommsemtr. 15, Dresden 01069, Germany, Fraunhofer Portugal Center for Smart Agriculture and Water Management AWAM, Parque de Ciencia e Tecnologia de Vila Real - Regia Douro Park, Vila Real 5000-033, Portugal

2023

Sensors and actuators, B. Chemical

Sensors and actuators, B. Chemical

ISSN:0925-4005
年,卷(期):2023.397(Dec.)
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