首页|Jiangnan University Reports Findings in Machine Learning (Rapid detection of six Oceanobacillus species in Daqu starter using singlecell Raman spectroscopy combined with machine learning)
Jiangnan University Reports Findings in Machine Learning (Rapid detection of six Oceanobacillus species in Daqu starter using singlecell Raman spectroscopy combined with machine learning)
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NETL
NSTL
Wiley
New research on Machine Learning is the subject of a report. According to news reporting out of Wuxi, People’s Republic of China, by NewsRx editors, research stated, “Many traditional fermented foods and beverages industries around the world request the addition of multi-species starter cultures. However, the microbial community in starter cultures is subject to fluctuations due to their exposure to an open environment during fermentation.” Financial support for this research came from National Key Research and Development Program of China. Our news journalists obtained a quote from the research from Jiangnan University, “A rapid detection approach to identify the microbial composition of starter culture is essential to ensure the quality of the final products. Here, we applied single-cell Raman spectroscopy (SCRS) combined with machine learning to monitor Oceanobacillus species in Daqu starter, which plays crucial roles in the process of Chinese baijiu. First, a total of six Oceanobacillus species (O. caeni, O. kimchii, O. iheyensis, O. sojae, O. oncorhynchi subsp. Oncorhynchi and O. profundus) were detected in 44 Daqu samples by amplicon sequencing and isolated by pure culture. Then, we created a reference database of these Oceanobacillus strains which correlated their taxonomic data and single-cell Raman spectra (SCRS). Based on the SCRS dataset, five machine-learning algorithms were used to classify Oceanobacillus strains, among which support vector machine (SVM) showed the highest rate of accuracy. For validation of SVM-based model, we employed a synthetic microbial community composed of varying proportions of Oceanobacillus species and demonstrated a remarkable accuracy, with a mean error was less than 1% between the predicted result and the expected value. The relative abundance of six different Oceanobacillus species during Daqu fermentation was predicted within 60 min using this method, and the reliability of the method was proved by correlating the Raman spectrum with the amplicon sequencing profiles by partial least squares regression.”
WuxiPeople’s Republic of ChinaAsiaCyborgsEmerging TechnologiesMachine Learning
NETL
NSTL
Wiley
