Abstract
By a News Reporter-Staff News Editor at Robotics & Machine Learning Daily News Daily News-New research on Biotechnology-siRNA- Based Therapy is the subject of a report. According to news reporting from Wuhan , People's Republic of China, by NewsRx journalists, research stated, "The incre asing emergence and re-emergence of RNA virus outbreaks underlines the urgent ne ed to develop effective antivirals. RNA interference (RNAi) is a sequence-specif ic gene silencing mechanism that is triggered by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs), which exhibits significant promise for antivira l therapy." The news correspondents obtained a quote from the research from the Chinese Acad emy of Sciences, "AGO2-dependent shRNA (agshRNA) generates a single-stranded gui de RNA and presents significant advantages over traditional siRNA and shRNA. In this study, we applied a logistic regression algorithm to a previously published chemically siRNA efficacy dataset and built a machine learning-based model with high predictive power. Using this model, we designed siRNA sequences targeting diverse RNA viruses, including human enterovirus A71 (EV71), Zika virus (ZIKV), dengue virus 2 (DENV2), mouse hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and transformed them into agshRNAs. We val idated the performance of our agshRNA design by evaluating antiviral efficacies of agshRNAs in cells infected with different viruses. Using the agshRNA targetin g EV71 as an example, we showed that the anti-EV71 effect of agshRNA was more po tent compared with the corresponding siRNA and shRNA. Moreover, the antiviral ef fect of agshRNA is dependent on AGO2-processed guide RNA, which can load into th e RNA-induced silencing complex (RISC). We also confirmed the antiviral effect o f agshRNA in vivo."
NETL
NSTL