摘要
一位新闻记者-机器人与机器学习的工作人员新闻编辑每日新闻-肿瘤学的新研究-胶质瘤是一篇报道的主题。根据Newsrx编辑在芬兰图尔库的新闻报道，研究表明：“福尔马林固定、石蜡包埋的(FFPE)组织切片通常用于癌症诊断、临床决策和生物库中。”本文利用自发拉曼光谱对46例已知甲基化亚型的FFPE组织进行了分子指纹图谱分析。我们的新闻记者从Turku大学的研究中引用了一句话：“Spectrum were used to construction tumor/non-tumor,IDH1WT/IDH1mut,”本文利用支持向量机和随机森林技术对不同类型的胶质瘤进行分类,利用激发拉曼光谱对识别出的频率进行验证,并利用质谱和TCGA对生物学结果进行验证,提出了一种基于Raman Based PathOLogy of Magnous Glusteria,Raman Based PathOLogy of Magnous Glusteria,Raman Based PathOLogy of MagnFPE组织切片的光谱。我们的新APOLLO平台区分肿瘤和非肿瘤组织，并识别出与肿瘤组织中更强烈的DNA和蛋白质相对应的新的拉曼峰。APOLLO区分异柠檬酸脱氢酶1M突变体(IDH1mut)和野生型(IDH1WT)肿瘤，并鉴定出IDHmut胶质瘤中高丰度的胆固醇酯水平。此外，APOLLO在细、小、小胶质瘤中具有很高的鉴别能力临床相关的胶质瘤甲基化亚型，区分IDH1mut类型内CpG岛高甲基化表型（g-cimp）和g-cimp低分子表型。
Abstract
By a News Reporter-Staff News Editor at Robotics & Machine Learning Daily News Daily News-New research on Oncology - Gliomas is the subject of a report. According to news reporting out of Turku, Finland, by N ewsRx editors, research stated, "Formalin-fixed, paraffin-embedded (FFPE) tissue slides are routinely used in cancer diagnosis, clinical decision-making, and st ored in biobanks, but their utilization in Raman spectroscopy-based studies has been limited due to the background coming from embedding media. Spontaneous Rama n spectroscopy was used for molecular fingerprinting of FFPE tissue from 46 pati ent samples with known methylation subtypes." Our news journalists obtained a quote from the research from the University of T urku, "Spectra were used to construct tumor/non-tumor, IDH1WT/IDH1mut, and methy lation-subtype classifiers. Support vector machine and random forest were used t o identify the most discriminatory Raman frequencies. Stimulated Raman spectrosc opy was used to validate the frequencies identified. Mass spectrometry of glioma cell lines and TCGA were used to validate the biological findings. Here we deve lop APOLLO (rAmanbased PathOLogy of maLignant glioma) - a computational workflo w that predicts different subtypes of glioma from spontaneous Raman spectra of F FPE tissue slides. Our novel APOLLO platform distinguishes tumors from nontumor tissue and identifies novel Raman peaks corresponding to DNA and proteins that a re more intense in the tumor. APOLLO differentiates isocitrate dehydrogenase 1 m utant (IDH1mut) from wildtype (IDH1WT) tumors and identifies cholesterol ester l evels to be highly abundant in IDHmut glioma. Moreover, APOLLO achieves high dis criminative power between finer, clinically relevant glioma methylation subtypes , distinguishing between the CpG island hypermethylated phenotype (G-CIMP)-high and G-CIMP-low molecular phenotypes within the IDH1mut types."
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