首页|Bone-seeking tumor cells alter bone material quality parameters on the nanoscale in mice

Bone-seeking tumor cells alter bone material quality parameters on the nanoscale in mice

扫码查看
原文链接
  • NETL
  • NSTL
  • Elsevier
Bone metastases related to breast and prostate cancer present with multiple challenges and skeletal related events like fragility fractures impair the quality of life of the patients significantly. To determine local alterations in bone material quality with bone metastasis, we subjected murine tibial specimens, generated after intratibial injections of either RM1 prostate cancer cells or EO771 breast cancer cells into male and female mice respectively, to high-resolution imaging modalities. Small and wide-angle X-ray scattering showed unaltered mineral characteristics in the more osteosclerotic prostate cancer model, while the quantification of calcium weight percentage via backscattered electron microscopy determined minor differences along the perilacunar bone matrix. Further analyses of mineral and collagen characteristics were performed using Raman spectroscopy and focused ion beam electron microscopy. Our study indicates that alterations in nanochannel properties occur due to the presence of bone seeking tumor cells with more prevalent nanopores in the perilacunar matrix.

Bone material qualityBone metastasisMouse modelHigh-resolution imagingPerilacunar matrixRaman spectroscopyNanoporosity

Johannes Krug、Christine Plumeyer、Anton Davydok、Sofie Dragoun Kolibova、Nico Fischer、Xuan-Thanh Le-Phuoc、Martina Rauner、Praveer Sihota、Michaela Schweizer、Bjoern Busse、Imke A.K. Fiedler、Katharina Jaehn-Rickert

展开 >

Department of Osteology and Biomechanics, University Medical-Center Hamburg-Eppendorf, Hamburg, Germany||Interdisciplinary Competence Center for Interface Research (ICCIR), Hamburg Germany

Institute of Material Physics, Hereon Outstation at DESY, Helmholtz Zentrum Hereon, Hamburg, Germany

Department of Osteology and Biomechanics, University Medical-Center Hamburg-Eppendorf, Hamburg, Germany

Divisions of Endocrinology and Molecular Bone Biology, Department of Medicine Ⅲ & University Center for Healthy Aging, Technische Universitaet Dresden, Dresden, Germany

Core Facility of Morphology and Electron Microscopy, Center for Molecular Neurobiology, Hamburg, Germany

Department of Osteology and Biomechanics, University Medical-Center Hamburg-Eppendorf, Hamburg, Germany||Interdisciplinary Competence Center for Interface Research (ICCIR), Hamburg Germany||Mildred Scheel Cancer Career Center Hamburg, University Cancer Center Hamburg, University Medical-Center Hamburg-Eppendorf, Hamburg, Germany

展开 >

2025

10.1016/j.bioadv.2024.214060

Biomaterials Advances

Biomaterials Advances

ISSN：2772-9516
年,卷(期)：2025.167(Feb.)
  • 49