首页|Effects of Aluminum Oxide Nanoparticles in the Spinal Cord of Male Wistar Rats and the Potential Ameliorative Role of Melatonin
Effects of Aluminum Oxide Nanoparticles in the Spinal Cord of Male Wistar Rats and the Potential Ameliorative Role of Melatonin
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Aluminum oxide nanoparticles (Al_2O_3 NPs) are widely utilized in vaccine manufacturing and other medical preparations. Melatonin has numerous effects as an antioxidant and anti-apoptotic. The purpose of this study was to examine the beneficial impact of melatonin on Al_2O_3 NPs toxicity in the spinal cord. Forty male rats were divided into four groups: Group Ⅰ, the negative controls (received standard diet and distilled water); Group Ⅱ, Al_2O_3 NPs (received 30mg/kg bw Al_2O_3 NPs); Group Ⅲ, melatonin and Al_O3 NPs (received 30mg/kg bw Al_2O_3 NPs + 10mg/kg bw melatonin); Group Ⅳ, melatonin (received 10mg/kg bw melatonin). All treatments were administered daily for 28 days by gastric gavage. After that, all rats were sacrificed, then, the samples from different spinal cords were subjected to histopathological, biochemical, and immunohistochemical analyses. Al_O_3 NPs markedly elevated malondialdehyde and 8-hydroxydeoxyguanosine while inhibiting superoxide dismutase and catalase. Al_2O_3 NPs also induced histological alterations in both gray and white matter manifested by neuronal degeneration, vacuolation, axonal degeneration, ballooning, and fusion of myelin sheaths. Furthermore, immunohistochemical results displayed a strong positive expression of caspase-3. Conversely, melatonin significantly mitigated the effects of Al_2O_3 NPs by increasing the activities of antioxidant enzymes and inhibiting malondialdehyde and 8-hydroxydeoxyguanosine. Moreover, melatonin alleviated most histological alterations induced by Al_2O_3 NPs and reduced caspase-3 immunoreactivity. Collectively, melatonin could protect the spinal cord and mitigate Al_2O_3, NPs-induced neurotoxicity.
NETL
NSTL
