首页|D-tagatose biotransformation from lactose by lactase and recombinant Bacillus subtilis expressing L-arabinose isomerase, and the product separation, purification and crystallization

D-tagatose biotransformation from lactose by lactase and recombinant Bacillus subtilis expressing L-arabinose isomerase, and the product separation, purification and crystallization

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  • NETL
  • NSTL
  • Elsevier
D-Tagatose is a low-calorie rare sugar with notable physiological benefits. The recombinant Bacillus subtilis expressing L-arabinose isomerase (LAI) was constructed and used together with lactase for biotransformation of D-tagatose from cheap substrate of lactose. Under optimum conditions, 45.6 g/L D-tagatose was produced from 200 g/L lactose, achieving a conversion yield of 0.228 g D-tagatose/g lactose. D-Glucose produced by lactose hydrolysis was removed by anaerobic fermentation with Saccharomyces cerevisiae. Subsequently, the sugar solution containing D-galactose and D-tagatose was decolorized by using powdered activated carbon, desalinated by anion and cation exchange resin beds, and separated by chromatographic column, resulting in decolorization rate of 95.5 %, desalinization rate of 93.8 % and D-tagatose solution purity of 97.9 %. Finally, the separated d-tagatose solution was concentrated and crystallized, resulting in D-tagatose crystals with 99.9 % purity. In summary, this paper establishes a complete bioprocess for D-tagatose from lactose catalyzed by using recombinant B. subtilis and lactase. The methods developed in this study show promise for mass production of food-grade D-tagatose from the inexpensive substrate lactose.

D-tagatoseBacillus subtilisLactaseL-arabinose isomeraseSeparation and purification

Xin Wen、Huibin Lin、Guangwen Liu、Yuhang Ning、Yilin Ren、Can Li、Chengjia Zhang、Nannan Dong、Jianqiang Lin、Xin Song、Guoqiang Zhuang、Jianqun Lin

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State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, 266237, China||Binzhou Institute of Technology, Weiqiao-UCAS Science and Technology Park, Binzhou, 256606, China

Shandong Academy of Chinese Medicine, Jinan, 250014, China

State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, 266237, China

Qingdao Longding Biotech Limited Company, Qingdao, 266108, China

School of Biological Engineering, Qilu University of Technology, Jinan, 250353, China

Binzhou Institute of Technology, Weiqiao-UCAS Science and Technology Park, Binzhou, 256606, China

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2025

10.1016/j.fm.2025.104785

Food microbiology

Food microbiology

SCI
ISSN：0740-0020
年,卷(期)：2025.131(Oct.)
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