首页|Front-light Structure with Excitation Light Polarized for Enhancing Lensless Fluorescence Imaging

Front-light Structure with Excitation Light Polarized for Enhancing Lensless Fluorescence Imaging

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  • NETL
  • NSTL
  • Myu, Scientific Publishing Division
In this study, we introduce a high-performance front-light structure integrated with optical polarization control to improve low-contrast fluorescent imaging in lensless devices. Lensless fluorescence imaging is an advanced technology with the potential to enhance the performance of biological and neurological imaging. However, there are still challenges to achieving high image quality, particularly in low-contrast fluorescent signals due to light scattering and absorption. The performance of a low-fluorescence stripe diffractor, fabricated using Norland Optical Adhesive 63 (NOA 63), was evaluated in comparison to polydimethylsiloxane (PDMS) material as a light guide. The diffractor is designed to operate with a hybrid emission filter on the image sensor. As a result, by coupling a 450 nm laser with either P- or S-polarization, it was shown that NOA 63 is notable as a diffractor material. Notably, S-polarized light showed a great excitation intensity and an enhanced contrast in fluorescent bead imaging compared with P-polarized light. Furthermore, the system was validated for biological applications by successfully detecting fluorescent bead emission in agarose-embedded samples of various thicknesses, simulating brain tissue. These results emphasize the system's ability to capture low-intensity fluorescence signals from deep layers of biological tissue.

lensless imaging devicefluorescent imagingfront-light structurediffractorlight-polarized

Arphorn Promking、Yoshinori Sunaga、Yasumi Ohta、Ryoma Okada、Hironari Takehara、Makito Haruta、Hiroyuki Tashiro、Jun Ohta、Kiyotaka Sasagawa

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Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan

Institute for Research Initiatives, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan

Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan||Medilux Research Center, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan

Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan||Nara Advanced Imaging Technology, Co., Ltd., 8916-12, Takayama, Ikoma, Nara 630-0101, Japan

Institute for Research Initiatives, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan||Department of Opto-Electronic System Engineering, Faculty of Science and Engineering, Chitose Institute of Science and Technology, 758-65 Bibi, Chitose, Hokkaido 066-8655, Japan

Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan||Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

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2025

10.18494/SAM5600

Sensors and materials

Sensors and materials

SCI
ISSN：0914-4935
年,卷(期)：2025.37(4)
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