首页|Physical fields reverse FeS04-induced VBNC state in Listeria monocytogenes and facilitate ferroptosis
Physical fields reverse FeS04-induced VBNC state in Listeria monocytogenes and facilitate ferroptosis
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NETL
NSTL
Elsevier
Non-thermal sterilization methods are effectively in eliminating foodborne pathogens while preserving the appearance and quality of food. In this study, three physical fields, magnetic field (MF), ultrasound (US), and blue light (BL), were introduced for their efficacy in sterilizing FeSO_4-induced viable but non-culturable (VBNC) Listeria monocytogenes (L. monocytogenes) cells over a 4 h treatment. The Fe-MF treatment induced L. monocytogenes cells to form VBNC state, resulting in an 80.13 % reduction in culturable cells, with a 90.58 % survival rate. The ultrasound-assisted FeSO_4 (Fe-US) treatment reduced the VBNC cell population by 52.63 %. In contrast, the blue light-assisted FeSO_4 (Fe-BL) treatment reversed the VBNC state toward culturable state at the node of 3 h, and induced irreversible 100 % cell death at 4 h, with a 94.73 % decrease in viability and 68.2 % membrane damage. Additionally, Fe-BL treatment led to cell wrinkling and secretion aggregation. BL treatment alone compromised membrane permeability and triggered intracellular protein aggregation. Mechanistic investigations revealed that BL-assisted treatment disrupted the protective mechanism of L monocytogenes when induced by FeSCU to form VBNC state, compromised VBNC cell membranes, promoted intracellular Fe~(2+) accumulation, and induced a reactive oxygen species (ROS) burst and lipid peroxidation, ultimately leading to ferroptosis. Prote-omic analysis identified 240 upregulated and 376 downregulated differentially expressed proteins, highlighting significant changes in pathways related to ribosome biosynthesis (related genes rplJ and hpf significant upregulated), intracellular iron homeostasis (ctaB, hemN, and bno2590 downregulated), cellular morphology (mreB and tagH downregulated), oxidative stress response (lmo0720 and lmo0799 downregulated), and DNA synthesis (recA, dnaD, yidC2 and fruB downregulated). In conclusion, Fe-BL treatment effectively disrupted VBNC cell membranes, induced iron homeostasis imbalance, and triggered Fenton reaction-mediated ferroptosis. These findings provide a promising non-thermal sterilization strategy for inactivating VBNC L. monocytogenes, offering potential applications in food safety.
Blue lightFerroptosisFeSO_4Magnetic fieldUltrasoundSynergistic sterilization
Xiaolin Zhu、Yunhong Wang、Shurui Peng、Jiayi Zhang、Hongbo Li、Haizhen Mo、Liangbin Hu
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School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China
NETL
NSTL
Elsevier
