首页|Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera
Cryopreservation of Spix's yellow-toothed cavy epididymal sperm using Tris- and coconut water-based extenders supplemented with egg yolk or Aloe vera
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NSTL
Elsevier
Addressing the establishment of biobanks for the conservation of wild hystricomorph rodents? germplasm, we verified the effects of different extenders and distinct concentrations of non-permeant cryoprotectants on the sperm parameters of Spix's yellow-toothed cavies. Nine testis-epididymis complexes were used for sperm collection by retrograde washing using Tris or a powdered coconut water extender (ACP (R)-116c). Spermatozoa were diluted and frozen with the same extenders supplemented with egg yolk or Aloe vera at a 10% or 20% concentration. After recovery and cryopreservation, all samples were evaluated for sperm kinetic parameters, morphology, membrane integrity, osmotic response, and sperm-binding capability using an egg yolk perivitelline membrane assay. After recovery, no differences were observed between Tris and ACP (R)-116c that provided 515.4 x 10(6) sperm/mL and 561.6 x 10(6) sperm/mL, presenting >65% motile sperm, respectively. After cryo-preservation, most effective preservation of sperm kinetic parameters (68.1 x 5.9% motile sperm) and membrane integrity (48.2 x 7.4%) was provided by Tris extender supplemented with 10% egg yolk. However, both ex-tenders supplemented with any concentration of egg yolk or Aloe vera presented similar preservation of osmotic response and sperm-binding ability after cryopreservation. In summary, we suggest the use of a Tris extender supplemented of 10% egg yolk for cryopreservation of Spix's yellow-toothed cavy epidydimal sperm.
NSTL
Elsevier
