首页|HPLC Fluorescence Method for Eugenols in Basil Products Derivatized with DIBI
HPLC Fluorescence Method for Eugenols in Basil Products Derivatized with DIBI
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NSTL
Eugenols (Eugs) such as eugenol (Eug), methyleugenol (MeEug), and linalool (Lin) in basil product are the main bioactive components of basil products and have a terminal double-bond. A sensitive HPLC-fluorescence method for Eugs derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)iodobenzene (DIBI) was developed. Good separation of DIB-Eugs was achieved within 20min on an Atlantis T3 column (50 x 2.1mm i.d., 3 mu m) with a mobile phase of methanol-water. The calibration curves obtained with Eug standards showed good linearities in the range of 0.1-50 mu M (r>0.999). The limits of detection at a signal-to-noise ratio (S/N)=3 for Eug, MeEug, and Lin were 1.0, 6.0, and 4.8nM, respectively. The limits of quantitation (SIN= 10) of the Eugs were lower than 19.9 nM. The accuracies for the Eugs were within 96.8-104.6%. The intra- and inter-day precisions as relative standard deviations for the Eugs were less than 1.2 and 9.6% (n=3). The recoveries of Eug, MeEug, and Lin were 99.0 +/- 0.1, 98.0 +/- 0.2, and 96.0 +/- 0.4% (n =3), respectively. The DIB-Eugs were confirmed to be stable for 2h (>90%) at room temperature and 24h (>95%) at 4 degrees C. These parameters of the proposed method were useful for the simultaneous determination of Eugs in basil products. Therefore, the developed method may be a powerful tool for the quality evaluation of dried commercially available basil products.
NSTL
