首页|Multiplex SYBR Green real-time PCR for Lactobacillus acidophilus group species targeting biomarker genes revealed by a pangenome approach
Multiplex SYBR Green real-time PCR for Lactobacillus acidophilus group species targeting biomarker genes revealed by a pangenome approach
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
The Lactobacillus acidophilus group consists of seven closely related species. Among these, Lb. acidophilus, Lb. gallinarum, and Lb. helveticus help maintain gut health and are used as a starter for fermented food. However, these species are difficult to differentiate using conventional methods due to the high similarity between the 16S rRNA and housekeeping genes. Thus, in this study, we selected biomarker genes to identify and discriminate the three species via pangenome analysis, and a multiplex SYBR Green real-time PCR that can be detected simul-taneously in a single tube was developed. Pangenome analysis revealed three specific target genes: mucus-binding protein precursor to detect Lb. acidophilus, an amino acid ABC superfamily ATP binding cassette transporter carrier protein to detect Lb. gallinarum, and selenocysteine lyase to detect Lb. helveticus. The speci-ficity was robustly verified using 26 Lb. acidophilus group strains and 62 other strains. The detection limits were 10(1) colony-forming units (CFU)/ml in pure culture. As per our findings, the developed method satisfactorily monitored Lb. acidophilus group species in probiotic and dairy products. This result suggests that real-time PCR based on specific targets provides a promising approach for the rapid, accurate, and sensitive identification of these three species.
Lactobacillus acidophilus group speciesBiomarker genesMultiplex SYBR Green real-time PCRDetectionMelting temperaturePangenome analysisIDENTIFICATIONQUANTIFICATIONASSAY
Kim, Eiseul、Kim, Dayoung、Yang, Seung-Min、Kim, Hae-Yeong
NSTL
Elsevier
