International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：15.DOI:10.1007/s10989-022-10444-8

Atrial Natriuretic Peptide (ANP) Suppress lambda-Carrageenan-Induced Acute Paw Edema by Modulating Pro-inflammatory Genes in Swiss Albino Mice

Kalaiarasu, Lakshmi Priya Babu, Aadhil Ashwaq Krishnan, Kalaiselvi Dhanusu, Suresh Vellaichamy, Elangovan
International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：15.DOI:10.1007/s10989-022-10444-8
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Atrial Natriuretic Peptide (ANP) Suppress lambda-Carrageenan-Induced Acute Paw Edema by Modulating Pro-inflammatory Genes in Swiss Albino Mice

Kalaiarasu, Lakshmi Priya 1Babu, Aadhil Ashwaq 1Krishnan, Kalaiselvi 1Dhanusu, Suresh 1Vellaichamy, Elangovan1
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作者信息

  • 1. University of Madras,Univ Madras
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Abstract

The present study was designed to investigate the anti-inflammatory activity of atrial natriuretic peptide (ANP) using lambda-Carrageenan (lambda-CAN)-induced acute inflammation in the mice model. A single subplantar injection of lambda-CAN (1% in 0.9% saline) triggers acute paw edema. ANP (2 mu g/kg body weight/day/i.p) was given 30 min prior or after administration of lambda-CAN. The standard drug Aspirin (ASP) treated group was used as a positive control. A marked increase in paw thickness (32.56% p < 0.001) and thermal pain latency (0.82 +/- 0.03 vs 3.6 +/- 0.12; P < 0.001) were noticed in lambda-CAN-induced mice as compared with control. The level of pro-inflammatory marker genes such as Nuclear factor-kappa B and Cyclooxygenase-2 were significantly increased (p < 0.001 respectively) in lambda-CAN-induced mice as compared with control. A similar trend was noticed in pro-inflammatory cytokines such as Interleukin-6, Interleukin-1 beta, Tumour necrosis factor-alpha and Prostaglandin E2 (p < 0.001 respectively). On the contrary, ANP exhibits a marked reduction in paw edema thickness (pre-8.76% and post-9.12%, p < 0.001) and thermal pain latency in both pre-0.82 +/- 0.03 vs 1.2 +/- 0.06 and post-0.82 +/- 0.03 vs 1.6 +/- 0.02 (P < 0.001) were noticed in ANP pre-and post-treated group. Further myeloperoxidase activity, pro-inflammatory marker genes and pro-inflammatory cytokines were also reverted (p < 0.01 respectively) to near-normal levels. The present study results suggest that pre-and post-treatment of ANP positively neutralizes the lambda-CAN induced acute edematic and inflammatory responses. The anti-edematic and anti-inflammatory action of ANP was found to be much more effective than that of the non-steroidal drug aspirin (ASP).

Key words

Atrial natriuretic peptide (ANP)/lambda-Carrageenan (lambda-CAN)/Anti-inflammatory/Natriuretic peptide receptor-A (NPR-a)/Pro-inflammatory and Aspirin (ASP)/ALPHA PRODUCTION/TNF-ALPHA/KAPPA-B/NF-KB/INHIBITION/CYTOKINES/RECEPTOR/CELLS/DISRUPTION/EXPRESSION

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出版年

2022
International journal of peptide research and therapeutics.

International journal of peptide research and therapeutics.

ISSN：1573-3149
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