首页|Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine

Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine

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SARS-CoV-2 spread in humans results in continuous emergence of new variants, highlighting the need for vaccines with broad-spectrum antigenic coverage. Using inter-lineage chimera and mutation-patch strate-gies, we engineered a recombinant monomeric spike variant (STFK1628x) that contains key regions and res-idues across multiple SAR-CoV-2 variants. STFK1628x demonstrated high immunogenicity and mutually complementary antigenicity to its prototypic form (STFK). In hamsters, a bivalent vaccine composed of STFK and STFK1628x elicited high titers of broad-spectrum neutralizing antibodies to 19 circulating SARS-CoV-2 variants, including Omicron sublineages BA.1, BA.1.1, BA.2, BA.2.12.1, BA.2.75, and BA.4/5. Furthermore, this vaccine conferred robust protection against intranasal challenges by either SARS-CoV-2 ancestral strain or immune-evasive Beta and Omicron BA.1. Strikingly, vaccination with the bivalent vaccine in hamsters effectively blocked within-cage virus transmission of ancestral SARS-CoV-2, Beta variant, and Omicron BA.1 to unvaccinated sentinels. Thus, our study provided insight and antigen candidates for the development of next-generation COVID-19 vaccines.

Wu, Yangtao、Wang, Shaojuan、Zhang, Yali、Yuan, Lunzhi、Zheng, Qingbing、Wei, Min、Shi, Yang、Wang, Zikang、Ma, Jian、Wang, Kai、Nie, Meifeng、Xiao, Jin、Huang, Zehong、Chen, Peiwen、Guo, Huilin、Lan, Miaolin、Xu, Jingjing、Hou, Wangheng、Hong, Yunda、Chen, Dabing、Sun, Hui、Xiong, Hualong、Zhou, Ming、Liu, Che、Guo, Wenjie、Guo, Huiyu、Gao, Jiahua、Gan, Congling、Li, Zhixiong、Zhang, Haitao、Wang, Xinrui、Li, Shaowei、Cheng, Tong、Zhao, Qinjian、Chen, Yixin、Wu, Ting、Zhang, Tianying、Zhang, Jun、Cao, Hua、Zhu, Huachen、Yuan, Quan、Guan, Yi、Xio, Ningshao

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Xiamen Univ

Univ Hong Kong

Chinese Acad Med Sci

2022

Cell Host & Microbe

Cell Host & Microbe

SCI
ISSN:1931-3128
年,卷(期):2022.30(12)
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