首页|CRISPR/Cas 9 A novel genome editing technology for cotton improvement
CRISPR/Cas 9 A novel genome editing technology for cotton improvement
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NSTL
CRISPR-Cas9 is a valuable gene editing tool in plant science research. The presence of clustered repeats was first discovered by Ishino et al., (1987) when the loci containing repeat sequences with an unknown function found in the Escherichia coZi genome. Prokaryotes have CRISPR family throughout the genome was discovered by Mojka et at, (2000) and the name CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) was coined and designed as Cas genes by Jansen et al., (2002). First experimental evidence was reported for CRISPR adaptive immunity and the CRISPR complexes cleave the RNA and the tracr RNA forms a duplex structure with CrRNA in association with Cas 9 by Barrangou et al., (2007). The PAM (Protospacer Adjacent Motif) is a motif with sequence NGG (any, guanine, guanine) is specific to Streptococcus pyogenes and 52 -NAG (any, adenine, guanine) the origin of spacers and adaptive immune system was reported by Bolotin et al., (2005) and Pourcel et al., (2005). The CRISPR acts upon DNA targets and the spacers are converted in to mature crRNAs that act on small guide RNAs (Marrafini et al., 2008, Brouns et al., 2008). The Cas 9 is guided by spacer sequences and cleaves target DNA via Double Stranded Breaks (Garneu et al., 2010).
J. AMUDHA、G. BALASUBRAMANI
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ICAR-Central Institute for Cotton Research, Nagpur
NSTL
